UP TGT Biology 2016 Official Paper (Previous Year Paper)

Key Points

• The theory of evolution was described by Charles Darwin as descent with modification by natural selection.
• 'Descent with modification' explains how existing life forms can have a common ancestor as well as show variations.
• These variations were inheritable in nature i.e., they can be passed on from one generation to another.
• Some of these inheritable variations would enhance the survivability of some, enabling them to leave more progeny.
• These progenies would also survive better and hence give rise to new life forms.

Additional Information

• Inheritance of acquired characters was proposed by Lamarck, which states that physical characteristics of an organism acquired by use and disuse in a lifetime can be passed on to the next generation.
• The concept of struggle for existence refers to the competition for the available resources that are required for survival. Darwin suggested that there is a continuous struggle for existence in which only the fittest would survive.
• 'Like begets like' is a phrase that refers to the similarity of offspring with the parents due to inheritable characters.

Key Points

• Food chain - is a linear sequence of flow of energy takes place between different organisms that are related to each other by the food they consume.

• Food web - is an interconnection of different food chains in an ecosystem.

• Both food chains and food webs outline who eats whom in an ecosystem.

• The organisms are classified into different categories according to their feeding order.

• Producers - synthesize food from solar energy. This covers all photosynthesizing plants and algae.

• Primary consumers - come immediately after producers and comprise herbivores that feed on the producers directly.

• Secondary consumers - feed on primary consumers and hence comprise of carnivores.

• Decomposers - break down dead, decaying organisms into simpler inorganic substances. E.g. - Fungi.

Important Points

• Scavengers - are carnivorous organisms that feed on the dead and decaying flesh of an animal.
• They differ from other carnivores because they do not hunt.
• Examples - Vultures, Hyenas, Jackals, Crows.

Additional Information

• There are two main types of food chains:
• Grazing - These food chains start with the producers and are the major system of energy flow in aquatic ecosystems.
• Detritus - The starting point is dead organic matter from other food chains and is the major energy flow system in terrestrial ecosystems.

Key Points

• Gluconeogenesis is the process by which glucose is synthesized from non-sugar precursors like lactate, pyruvate, alanine and glycerol.
• Functionally, the process is antagonistic to the process of glycolysis where glucose is broken down to form products like pyruvate, alanine, etc.
• It mainly occurs in the liver after long periods of fasting when alternative sources of glucose are required.

Important Points

• Both alanine and pyruvate are converted to pyruvate.
• Pyruvate enters the mitochondria and is converted to oxaloacetate (OAA).
• OAA may enters the TCA cycle to form malate, which can be transported back to cytoplasm.
• Malate is converted to OAA which is then decarboxylated and phosphorylated by the PEP carboxykinase.
• Phosphoenol pyruvate (PEP) enters the gluconeogenic cycle, which is reverse glycolysis to yield glucose-6-phosphate.
• Glycerol is released into the plasma by the adipose tissues and is taken up by the liver.
• Glycerol is converted to dihydoxyacetone phosphate (DHAP) which enters the gluconeogenic pathway.

Additional Information

• Glycolysis - is the process by which glucose is utilized by the cell for energy production. It takes place in the cytoplasm.
• Glycogenolysis - is the specific biochemical pathway by which glycogen in the liver cells is broken down into glucose.

Key Points

• Ascaris is an endo-parasitic nematode that causes Ascariasis.
• It is found in the intestine of infected humans.
• Infection is caused by ingestion of infective eggs from contaminated soil.
• The larvae hatch and penetrate into the intestinal mucosa.
• They are then carried to the liver and from there to the heart and lungs.
• The larvae enlarge and rupture in the alveoli.
• They get coughed up and then swallowed.
• They reach the small intestine and mature thereby moulting.
• Moulting is the process of shedding the outer layer or covering when a replacement layer is formed.

Key Points

• The earliest evidence of social life among early humans is typically attributed to Neanderthal man.
• Neanderthals, belonging to the species Homo neanderthalensis, are known for their relatively complex social structures and behaviors, which included caring for the sick and elderly, burying their dead, and possibly engaging in rituals, all indicating a form of social life.
• Cro-Magnon men (early modern humans) also exhibited advanced social structures, but they appear later in the archaeological record compared to Neanderthals.
• ‘Cro-Magnon Man’ is the name given to the early humans who existed 40,000-10,000 years ago.
• The name came from the five skeletons that were found from a rock shelter in France, which was known by the same name.
• They have been recently renamed as Early Modern Human because it was found after much research that they do not have significant differences from modern humans.
• They are our immediate ancestor hominin who were in the process of developing the modern human behaviours.
• The physical differences between Cro-Magnons and modern humans are key to the change in lifestyle from long-distance hunting to sedentism, which requires them to live in groups and thus have a social life.
• They are also known to have used modern tools and had burial rituals.

Additional Information

• Java man – refers to an extinct hominin of the species Homo erectus that was discovered from Java. The thighbones discovered indicated their upright posture.
• Peking man – refers to Homo erectus fossils that were found from Zhoukoudian site of China. They lived about 2 million – 250,000 years ago.

Concept:

A vestigeal organ refers to those organs or parts of the body that have lost their functions or just retain minor ones. They were functional in our ancestors because of some necessity that has become non-existent over time.

Key Points

• Wisdom tooth - is the 3^rd molar in our dentition which has become vestigeal because of change in our diet. It is believed that it was present in our ancestors to help them chew raw, course food better.
• Appendix - is a finger-like pouch attached to the large intestine and is believed to have been used for digestion in our ancestors with a more herbivorous diet.
• Tail bone - is a bone at the end of the spine and is thought to be the remnant of a pre-existing tail. It is also known as coccyx.
• Bones - are not vestigeal as they form the skeletal structure of our body and is required for support and movement.

Concept:

• Alimentary canal is a hollow tubular organ that helps in the digestion of ingested food.
• It may be of two types:
• Complete alimentary canal – has 2 separate openings, mouth for ingestion and anus for egestion.
• Incomplete alimentary canal – has only one opening which functions for both ingestion and egestion.

Key Points

• Taenia refers to a genus of tapeworms belonging to phylum Platyhelminthes.
• In some organisms like Taenia, the alimentary canal is absent altogether.
• Taenia is an endoparasite in which digestion takes place by absorbing the nutrients from the host directly through their body surface.

Additional Information

• Fasciola – or liver fluke has an incomplete alimentary canal with a single ventral mouth.
• Ascaris – or roundworm has a complete alimentary canal with an anterior mouth and posterior anus.
• Ancylostoma – or hookworm has a complete muscular alimentary canal.

Key Points

• Crassulacean Acid Metabolism or CAM is a pathway for carbon fixation that is found in some plants.
• It is so-called because it was first found in the Crassulaceae family plants.
• It is a special adaptation for conserving water in xeric plants (that are found in dry conditions).
• In this process, the stomata open only at night and remain closed during the day.
• At night, the CO₂ is converted into malic acid which is stored in the vacuole.
• During daytime as the atmospheric CO₂ is absent, the malic acid is transported out of the vacuole and converted to pyruvate and CO₂. This CO₂ then takes part in the Calvin-Benson cycle in the chloroplast.

Concept:

• Cell cycle comprises of 2 phases:
• M-phase - represents the actual cell division phase where mitosis occurs.
• Interphase - represents the time in between two M-phases and lasts for more than 95% of the cell cycle.
• During Interphase, the amount of DNA is doubled by the process of DNA replication without increasing the number of chromosomes in a cell.

Important Points

• Sister chromatids are identical copies of the same chromosome and are formed by DNA replication during interphase.
• They are held together by a structure called centromere.
• They can be distinctly observed during Metaphase when the condensation of chromosome is completed.
• The sister chromatids separate by moving towards opposite ends of a dividing cell.
• By the end of divisional phase two daughter cells are formed with same amount of DNA as the parent cell.

Additional Information

• Homologs - Homologous chromosomes refer to the pair of chromosomes where one comes from the mother and another from the father. They are similar in size, centromere position and gene loci.
• Bivalents - refer to the replicated homologous chromosomes, where an exchange of DNA takes place among two non-sister chromatids by forming a synapse.
• Alleles - refer to the variant forms of a gene, out of which an individual inherits two alleles for a particular gene. The interaction between the two allelic forms determine the phenotype associated with that gene.

Key Points

• Soil is composed of soil particles that are of 3 major types depending on their sizes:
• Sand - are the largest particles.
• Silt - are intermediate in size.
• Clay - are the smallest particles.
• The different proportions of these soil particles give different textures to the soil.
• Soil is categorized into different types based on their textures. The main types include sandy, silty, clay and loamy soil.
• From the given figure we can see that loamy soil is composed of about 50% sand and 50% of silt and/or clay.

Key Points

• Exocoetus is a genus that belongs to the family Exocoetidae.
• They are called flying fish because they can jump out of the water surface and remain airborne for some time.
• They have a torpedo-like body shape that helps them to break water surface with high speed.
• They also have large wing-like pectoral fins that help them in gliding and being airborne.
• This 'flying' ability helps them to escape the predators that follow them.

Additional Information

• Sharks - are a group of cartilaginous fishes that are found all over the world.
• Chimera - are cartilaginous fishes that are also called ghost sharks. and are related to sharks and rays.
• Latimeria - is the genus with the only living species of coelacanth, found along the coasts of the Indian Ocean.

Key Points

• Metamorphosis – is the process by which an animal undergoes a rapid change in form and structure after birth to form an adult that is significantly different from its juvenile stages.
• Retrogressive metamorphosis – refers to the type of metamorphosis that involves degenerative changes, meaning the juvenile phase lose some advanced characters in the process of development to become an adult with more primitive characters.
• It is found in Urochordates like Herdmania, where larva shows chordate characters that are lost during development into the adult.

Important Points

• Changes involved during retrogressive metamorphosis:
• Free-swimming active larva changes into sedentary inactive adult.
• Larval tail with caudal fins shortens and disappears.
• Nerve cord and notochord break down and are consumed by phagocytes.
• Adhesive papillae disappear.
• Larval sense organs, ocellus and otolith, are lost.
• Sensory vesicles changes into adult cerebral ganglion.

Key Points

• Pectoral and pelvic girdles represent the shoulder and hip joints respectively.
• These joints usually have a ball and socket type of joint to allow for a range of movement.
• These joints have a rounded ball-like bone end that fits into a cup-like end of another bone.

Important Points

• Both the pectoral and pelvic girdles in a frog have 2 equal halves called ‘ossa innominata’ and consist of 3 bones each.
• The pelvic girdle has a depression called acetabulum at the junction of the ilium, ischium, and pubis bones. The head femur of the hind limb articulates into the acetabulum and forms a ball and socket structure.
• The pectoral girdle has a concavity called glenoid cavity formed at the junction of the scapule and coracoid bones. The head of lumerus of the forelimb articulates into the cavity and forms the ball and socket joint.

Additional Information

• The joints are divided into 3 classes:
• Fibrous Joint - are held together by fibrous connective tissue and do not show any movement. E.g.- Skull bones.
• Cartilaginous Joint - where the bones are connected by cartilages and allow little movement. E.g.- Joints between vertebrae.
• Synovial Joint - with fluid-filled cavity in between the bones that allows maximum movement. E.g.- Knees, shoulders.
• Synovial Joints are again of the following 6 types:
• Hinge - is found in elbow.
• Pivot - is found in first and second vertebrae of neck.
• Saddle - is found in the thumb joint.
• Ball and Socket - is found in shoulder and hip joints and allows maximum flexibility.
• Condyloid/Ellipsoidal - is found in the joints of wrist and fingers.
• Planar/Gliding - is found in the carpal bones in hand.

Key Points

• Pheretima is a genus of earthworms that belong to the phylum Annelida.
• The blood of Pheretima is red in colour due to the presence of haemoglobin, which is an iron-containing pigment.
• It differs from vertebrate blood because haemoglobin is not contained in the corpuscles, rather present dissolved in the plasma.
• It is called intercellular haemoglobin as it is present between the cells (corpuscles) in the plasma.
• The blood corpuscles are colourless and produced in the blood glands along with haemoglobin.
• Blood glands are located in the 4^th, 5^th and 6^th segments.

Additional Information

• In vertebrates like humans, haemoglobin is present in the erythrocytes in the blood and hence called intracellular haemoglobin.
• It serves the function of binding oxygen molecules and transporting them to the tissues.
• Haemocyanin is a copper-containing respiratory pigment that helps in transporting oxygen in arthropods and molluscs and turns blue on oxygenation.

Key Points

• Genophore is the term used for prokaryotic (bacterial) DNA.
• The term ‘prokaryotic chromosome’, although used, is misleading because it does not contain chromatin.
• Bacterial DNA is condensed by the process of supercoiling only and not formation of chromatin.
• Genophores are usually circular in structure, rarely linear.
• They are much smaller in size than Eukaryotic chromosome.
• They are present in the nucleoid region of the cell.

Additional Information

• Prokaryotic cells do not have a true nucleus and membrane-bound organelles.
• The genetic material is present in a specific region of the cell called nucleoid.
• They have a cell wall and may also have an additional layer called capsule.
• They may have pili or fimbriae that help them to attach to surfaces.
• They also have flagella to help them in locomotion.
• They have 70s type of ribosome.
• Some bacterial cells also have an extra-chromosomal DNA called plasmids that contain antibiotic resistance genes.

Key Points

• Marchantia is a liverwort that is considered to be one of the most primitive bryophytes.
• It is a heterothallic plant with the antheridiophore and archegoniophore on different thalli.
• They reproduce sexually to form the sporophyte.
• The zygote develops to form the sporophyte which is differentiated into:
• Foot - It is the basal swollen part that helps in anchoring and gives nutrition to the sporophyte.
• Seta - It is the stalk that connects the foot to the capsule.
• Capsule - It is an enlarged oval structure with a single jacket layer, which encloses the sporogenous tissue in young sporophytes.
• The sporogenous tissue differentiates into:
• Spore mother cells - undergo meiosis to form the haploid spore tetrad.
• Elater mother cells - give rise to diploid and sterile elaters.

Important Points

• Elaters are specialized structures of liverworts that help in spore dispersal.
• They are tubular, non-sporogenous cells with spiral wall thickenings and are found inside the capsules.
• They are hygroscopic in nature, meaning they respond to the changes in moisture content.
• The movement of elaters loosens the spore mass and leads to the scattering of spores in the air.
• They are sterile diploid cells that are produced in the sporophyte along with the spores.
• Elaters are diploid because they are formed by differentiation and without any further division.
• On the other hand, spores are formed after meiosis and thus become haploid.

Additional Information

• Elaters are absent in mosses.
• Elaters present in hornworts are called pseudo-elaters because they are functionally similar but structurally different from that of the liverworts.

Key Points

• Thomas Robert Malthus was an English economist who proposed the famous Malthusian theory of population growth.
• According to this theory, a population grows exponentially while the resources to sustain that population remains linear, resulting in a calamity that reduces back the population.
• Darwin wrote the book 'On the Origin of Species' in which he explained the theory of evolution by natural selection as 'descent with modification'.
• The Malthusian theory influenced Darwin’s theory of evolution where he stated that there exists a ‘struggle for existence’ in nature and ‘survival of the fittest’ takes place.
• ‘Struggle for existence’ refers to the competition for available resources required for survival.

Additional Information

• A. R. Wallace -
• Alfred Russel Wallace was also a naturalist, biologist and geographer.
• He worked with Charles Darwin on the theory of evolution.
• His contributions are not just in science but also in socialism and spiritualism.
• H. Spencer –
• Herbert Spencer was a philosopher, anthropologist, biologist who is known for his hypothesis on social Darwinism.
• He stated that social evolution takes place much like biological evolution where the same principles of natural selection and ‘survival of the fittest’ can be applied.
• H. de Vries – 
• Hugo de Vries was a botanist and geneticist, known for his mutation theory of evolution.
• He suggested that speciation (evolution of species) occurs due to sudden large changes in traits rather than small gradual changes, as was previously suggested by Darwin.

Key Points

• Cleavage refers to the series of mitotic divisions that divides an embryo after fertilization.
• The egg cytoplasm is divided into smaller nucleated cells called blastomeres.
• 16-64 blastomeres form the morula stage.
• Cleavage is mainly of 2 types:
• Holoblastic – is a complete cleavage where the cleavage furrow goes all the way through the egg due to less amount of yolk.
• Meroblastic – is an incomplete cleavage where the furrow does not go through the complete egg due to high amount of yolk.

Important Points

• The first division starts at the animal pole and moves towards the vegetal pole of the egg.
• The egg yolk in a frog is concentrated in the vegetal hemisphere which causes the second division to start at the animal pole before the first furrow goes through the vegetal hemisphere.
• The first two divisions are at right angles to each other, while the third is equatorial.
• However, the furrow is slightly displaced from the equator because of the vegetally placed yolk.
• This results in an unequal frog embryo with 4 small blastomeres (micromeres) and 4 large blastomeres (macromeres).

Additional Information

Key Points

• The given terms are in relation to the various forms in which water is available in the soil.
• Hollard – is the total water content present in the soil.
• Chresard is the amount of water available for the plants in the soil.
• It is also called growth water or available water.
• It contains about 75% of capillary water because plant roots cannot absorb water from capillaries less than 0.2µm in diameter.
• Echard – is the part of Hollard that is unavailable for the plants. It consists of hygroscopic water, chemically combined water, water vapour and 25% of capillary water.

Additional Information

The soil water can be of the following types:

• Gravitational water – reaches the water table after rainfall due to gravity. It is not available to plants but can be obtained mechanically by pumps, etc.
• Hygroscopic water – is the thin film of water that is held together by soil particles and is also not available for plants.
• Chemically combined water – refers to the water molecules bound to chemical substances present in the soil.
• Capillary water – is present in the narrow pores between the soil particles and is the only available water for plant absorption.

Key Points

• K. C. Mehta – 
• Karam Chand Mehta is known for his contribution to plant pathology.
• He worked on the recurrence of wheat rusts in the plainlands of India.
• He found that though the rust spores were killed by the summer heat, new spores were blown in from the hills causing fresh infections every year.
• B. B. Mundkar – 
• Balchandra Bhavanishankar Mundkur was an Indian mycologist and plant pathologist.
• He founded the Indian Phytopathological Society.
• R. S. Singh – 
• He is the author of many books on plant pathology and plant diseases.
• E. J. Butler – 
• Sir Edwin John Butler was an Irish mycologist and plant pathologist who became the Imperial Mycologist in India.
• He is noted for identifying 150 species of plant pathogenic fungi.

Additional Information

• Wheat rust is a fungal disease caused by Puccinia species.
• They are recognised by rusty-coloured pustules on the plant surface.
• There are 3 types of rusts:
• Leaf rust – caused by P. recondita, is also called brown rust.
• Stem rust – caused by P. graminis, is also called black rust.
• Stripe rust – caused by P. striiformis, is also called yellow rust.

Key Points

• Volvox is a green alga that belongs to the class Chlorophyceae.
• It forms colonies on the surface of fresh-water ponds or lakes.
• The thallus, called coenobium, is characteristically hollow, spherical or oval, and has a fixed number of cells that is specific to the species.
• The cells are arranged in a single layer on the periphery, whereas the central part remains mucilaginous.
• The colony is polarized as the anterior cells have larger eyespots and the posterior cells reproduce on maturity.
• Both asexual and sexual reproduction takes place in Volvox.

Important Points

• The plakea stage is found during asexual reproduction in Volvox colonies.
• During asexual reproduction, the posterior reproductive cells enlarge many times to become gonidia.
• These gonidia become rounded from the pyriform cells, have increased pyrenoids, lose the flagella and eyespots.
• The gonidia are pushed to the centre and the first division takes place longitudinally with respect to the coenobium.
• The second longitudinal division is at right angle to the first and results in the 4-celled stage.
• The third longitudinal division results in the 8-celled plakea stage with 4 central cells and 4 peripheral cells.
• The plakea stage looks like a curved plate with the concave side facing the exterior.
• From here the 16-celled stage is formed as a hollow sphere with an opening called phialopore towards the exterior.
• The cells keep dividing until the characteristic number of that species, with the pointed ends of each cell directed inwardly.
• After this, inversion of the colony takes place such that the pointed ends of the cells become outwardly.
• The inversion starts with a constriction opposite the phialopore such that the cells get pushed out of the phialopore and form a spherical colony.
• These are known as the daughter colonies that may later separate from the parent colonies.

Key Points

• Selaginella is a pteridophyte that is commonly known as spike-moss.
• Rhizophore is a distinctive morphological feature of Selaginella.
• It is defined as a leafless structure that arises at the point of branching and grows downwards.
• They branch out at the tip when they come in contact with hard surface.
• The morphology of rhizophore is quite unique because it is considered as both stem and root.

Additional Information

• The root-like features of rhizophore:
• It is positively geotropic.
• The anatomy is similar to a root.
• The apex of rhizophore shows a root cap-like structure.
• Auxin transport in rhizophore is similar to that of root.
• The stem-like features are:
• Root hairs are absent.
• Rhizophore arises exogenously whereas root arises endogenously.
• Under controlled conditions, it can be induced to grow into a shoot.

Key Points

• Glycolysis is a metabolic pathway by which glucose is converted into pyruvate, which acts as a precursor for for other reactions.
• It is also known as the Embden-Meyerhof-Parnas (EMP) pathway.
• It takes place in the cytoplasm of the cell.
• The pathway involves partial oxidation of one 6-carbon glucose molecule to form two 3-carbon pyruvate molecules, which may then be utilized in the TCA cycle or anaerobic respiratory processes.
• It can take place in both aerobic and anaerobic conditions.

Mistake Points

• The glycolysis process itself does not require oxygen.
• But it is a vital process for cellular respiration.
• Hence, it takes place in both aerobic and anaerobic organisms.

Important Points

• The glycolytic pathway consists of 10 steps of enzymatic reactions, which can be divided into 2 phases - 
• Preparatory or Investment phase - 2 ATP molecules are utilized in this phase.
• Pay-off phase - 4 ATP, 2 NADH and 2 pyruvate molecules are produced in this phase.
• Phosphofructokinase is the rate-limiting enzyme of the pathway, meaning it determines the rate at which glycolytic reactions take place.
• Under aerobic conditions, the pyruvate enters the mitochondria for oxidative phosphorylation.
• Under anaerobic conditions, the pyruvate stays in the cytoplasm and gets converted to lactate or ethanol.

Key Points

• Palaemon is a genus of shrimp that belongs to the phylum Arthropoda.
• There are 19 pairs of appendages: first 5 are cephalic, next 8 are thoracic and the rest 6 are abdominal.
• These appendages are typically found on each segment of the body.
• Each of the first two segments have a pair of antennae or feelers.
• The first antenna is known as the antennule which lies behind the compound eye.
• The statocyst is present is the antennule appendage.

Important Points

• A statocyst is a sensory organ containing statolith and sensory hairs.
• The statolith is made up of calcareous bodies, which are grainy structures that lie centrally in the statocyst.
• The sensory hairs line up the statocyst.
• It is found in some aquatic invertebrates like shrimps.
• It helps the organism in balance and orientation with respect to gravity.
• The statocyst lies at the base of each of the two antennules.
• In prawns, the statocyst is present in the precoxa of the antennule.

Additional Information

• The various appendages of Palaemon are:
• 2 pairs of antennae - First antenna or antennule and second antenna.
• 1 pair of mandible - used for crushing food.
• 2 pairs of jaws - First maxilla or maxillula and second maxilla.
• 3 pairs of maxillipedes - The first two are flat and the third is elongated.
• 5 pairs of walking legs - The first two have pincers and are called chelate legs and are used for defence.
• 6 pairs of abdominal appendages - are called pleopods or swimmerets and are used for swimming.

Key Points

• The nucleosome model was proposed by Roger Kornberg for the DNA packaging in eukaryotic chromosome.
• Under electron microscopes, chromosomes were found to look like 'beads on a string' because of the series of spherical particles connected by a fine filaments.
• These 'beads' were termed as nucleosomes.
• Nucleosome is a complex of DNA and Histone proteins to condense the DNA into smaller volume inside the nucleus.

Important Points

• Histones are positively charged, basic proteins which are rich in Lysine and Arginine residues.
• The histones H2A, H2B, H3 and H4 are involved in the nucleosome structure, while the H1 protein acts like a linker molecule between two nucleosomes.
• 2 molecules of H3 and H4 form a core of tetramer (as a pair of dimers).
• This (H3)₂.(H4)₂ tetramer is associated with 2 independent dimers of H2A-H2B.
• DNA being negatively charged, wraps around the histone octamer and forms the nucleosome.
• DNA enters and leaves the same side and the two turns are sealed by the H1 protein.
• A nucleosome contains about 200bp of DNA, out of which 146bp is involved in the coiling of octamers and the rest is associated with the linker molecule.
• It forms the 11nm wide interphase fibre.

Additional Information

• Nucleosomes are the first level of DNA condensation involved in DNA packaging.
• Nucleosomes are further condensed into the 30nm solenoid which is again organized into the tightly packed metaphase chromosomes.
• Non-histone proteins (NHP) can also bind to DNA and are present in the nucleus, but they do not play any role in nucleosome formation.
• NHPs may have various functions related to higher level of DNA packaging.

Key Points

• Centrifugation is a method by which particles of different sizes and densities present in a solution can be separated by spinning them at high speed.
• This is achieved by spinning the solution around an axis to create a centrifugal force, that helps in sedimentation of the particles according to their size, shape or density.
• Anticoagulants or blood thinners are chemical substances that prevent the blood from clotting by preventing the synthesis or function of various clotting factors of blood.
• Anticoagulants are used in the laboratory so that human blood does not clot outside the human body.

Important Points

• The process by which different components of the blood are separated is known as blood fractionation.
• In this process, the blood sample is first mixed with an anticoagulant to prevent blood clots.
• The solution is then centrifuged such that 3 distinct layers are formed.
• The red blood cells or erythrocytes settle at the bottom, the white blood cells or leukocytes form the middle layer along with the platelets and the plasma remains at the top.
• This separation happens due to the difference of densities of the components.
• Erythrocytes settle at the bottom because they have denser cytoplasm due to the iron molecules of haemoglobin, which is present in the erythrocytes.
• The middle layer of leukocytes and platelets is greyish in colour and is called buffy coat.

Additional Information

• Erythrocytes - or red blood cells (RBCs) are the haemoglobin-containing cells in blood that transports oxygen and carbon dioxide to and from tissues.
• Leukocytes - or white blood cells (WBCs) are the cellular component of blood responsible for carrying out immune functions in our body.
• Platelets - or thrombocytes are another blood component that plays vital role in blood clotting and thus preventing loss of blood from damaged blood vessels by plugging it with a clot.
• Plasma - is the liquid component of blood that contains all the other blood components along with salts and enzymes. It helps in transporting nutrients, hormones or waste substances from one part of body to another.

Key Points

• George Bentham and Joseph Dalton Hooker proposed the natural system of classification in the book 'Genera Plantarum'.
• This system of classification is based on the morphological and anatomical features and the affinities between them.

Important Points

• They divided the plant kingdom into 2 divisions:
• Cryptogamia - included the non-flowering plants.
• Phanerogamia - included the flowering plants and was further divided into classes.
• These classes included Dicotyledons and monocotyledons.
• Dicotyledons were categorized into Series and then into Cohorts.
• Cohorts were further categorized into orders.
• According to the present-day classifications, the cohorts represent Order and the orders represent the Family.
• Example -Presently, Malvaceae is a family under order Malvales. But in Bentham-Hooker classification, Malvaceae was an order under the cohort Malvales.

Key Points

• Golgi apparatus is named after its discoverer Camillo Golgi.
• The Golgi apparatus is a membrane-bound organelle in the cell that acts as a factory for processing, packaging and transport of proteins.
• In plant cells, Golgi apparatus is termed as dictyosomes.
• It consists of flattened sac-like structures called cisternae and the associated vesicles.
• Usually 3-8 parallel sacs are stacked upon each other.
• It is a polarized structure with a forming face or the cis-face and a maturing face or the trans-face.
• The cis-face is convex and towards the nucleus and it receives the proteins from the Endoplasmic Reticulum(ER).
• The proteins move through the Golgi apparatus and get modified and sorted for transport to their destination.
• The proteins then exit from the trans-face and are carried by the secretory vesicles.
• The proteins are delivered to the intra-cellular targets or secreted outside the cell.

Additional Information

• Lysosome - is known as the suicidal bag of the cell because it helps in digestion or breaking down of macromolecules or foreign particles.
• Rough Endoplasmic Reticulum - contains ribosomes on their surface that helps in protein synthesis.
• Smooth Endoplasmic Reticulum - helps in carbohydrate metabolism.

Key Points

• Oxygen Dissociation Curve (OCD) is the sigmoid curve obtained by plotting percentage saturation of haemoglobin with O₂ against the partial pressure of O₂.
• Haemoglobin is the red-coloured iron-containing pigment present in the RBCs.
• When O₂ binds with haemoglobin, it is called oxyhaemoglobin.
• Binding of O₂ with haemoglobin is related to the partial pressure of oxygen.
• Partial pressure refers to the pressure contributed by an ideal gas in a mixture of gases.
• Partial pressure is represented as pO₂ for oxygen and pCO₂ for carbon dioxide.
• The S-shaped curve that is formed is known as sigmoid curve.

Important Points

• The factors that affect this binding are: partial pressure of O₂, partial pressure of CO₂, hydrogen ion concentration and temperature.
• The ODC is useful in studying the effects of these factors on the oxygen-binding of haemoglobin.
• In the alveoli, pO₂ is high, pCO₂ is low, H⁺ concentration is less and temperature is low and this favours the formation of oxy-haemoglobin.
• In the tissues, the conditions are reversed such that dissociation of oxygen from haemoglobin becomes favourable.​

Additional Information

• CURVE SHIFT TO LEFT -
• This indicates increase in affinity between oxygen and Hb.
• Due to increase in pH.
• Due to decreased temperature.
• Due to decrease in carbon dioxide.
• CURVE SHIFT TO RIGHT -
• This indicates decrease in affinity between oxygen and Hb.
• Due to decrease in pH.
• Due to increased temperature.
• Due to increase in carbon dioxide.

Key Points

• Unio is a freshwater bivalve mussel that belongs to the phylum Mollusca.
• As they have aquatic habitat, respiration occurs through gills known as ctenidium.
• The ctenidium is present in the mantle cavity which also protects it on both sides of the body.
• The mantle also prevents the clogging of ctenidium with silt and allows water current to pass in and out.
• The ctenidia divide the mantle cavity on each side into two chambers: infra-branchial chamber and supra-branchial chamber.
• Ctenidia refers to the elongated pair of gills present one on each side of the body.
• Each ctenidium is made up of two gill plates or laminae which are derived from the folding of the ctenidium.
• Each gill plate consists of two lamellae or flaps which are made up of parallel gill filaments.
• The space between two lamellae is divided by the interlamellar junctions into compartments called water tubes.
• The adjacent gill filaments are joined by interfilamentar junctions that contain small apertures called ostia.

Important Points

• During respiration, the water moves in through the inhalant siphon into the infra-branchial chamber, from where it passes into the water tubes through the ostia.
• From the water tubes, the water is passed to the supra-branchial chamber and then released out through the exhalant siphon.

Additional Information

• Protobranch – is a small, leaf-like, primitive type of ctenidia.
• Fillibranch – W-shaped gill filaments hang downwards into the mantle cavity, but the terminal ends bend upwards.
• Eulamellibranch – is the most advanced type of ctenidium in which W-shaped filaments have lateral cross-partitions to create water-filled cavities.
• Septobranch – are present transversely across mantle cavity to form a partition.

Key Points

• The ovule is the plant structure that develops into seed after fertilization.
• The ovule consists of the nucellus, integuments, micropyle and funicle.
• The integuments are the tissue layer that later develops into seed coat.
• The small opening in the integument called micropyle allows the pollen tube to enter the embryo sac where the male gametes are discharged.
• The ovule is attached to the base by the funiculus.

Important Points

• The endothelium is an additional layer of cells, formed after differentiation from the inner integument of ovule.
• It is sometimes referred to as the 'integumentary tapetum'.
• After fertilization, the endothelium separates from the integument.
• Without fertilization, the endothelium proliferates to form a 'pseudo-embryo'.
• These pseudo-embryos are often found in parthenocarpic fruits.
• It is found in the plants of Solanaceae family. E.g. - Tomato.

Key Points

• Thomas Hunt Morgan was an American biologist and embryologist who is known for his contribution towards genetics.
• Morgan's experiments verified the chromosomal theory of inheritance as suggested by Sutton and Boveri.
• Sutton and Boveri had suggested that pairing and separation of a pair of chromosome would lead to the segregation of the pair of alleles that they carried.
• Morgan experimented with Drosophila to establish that the basis of variation was caused by sexual reproduction.
• He further showed that when 2 genes in a dihybrid cross were located on the same chromosome, the proportion of parental gene combinations were much higher than the non-parental type. This was attributed to linkage of genes.
• Morgan found some genes to be tightly linked and some to be loosely linked.
• He received the Nobel Prize for Physiology or Medicine in 1933 for his discovery of 'hereditary transmission mechanisms in Drosophila'.

Important Points

• Drosophila melanogaster or fruit-fly was used as a model organism because of the following reasons:
• Their size (3mm) is small enough to keep millions in a laboratory, but not so small that cannot be seen without a microscope.
• They can be grown on simple synthetic media in the laboratory.
• They complete their life cycles in about 2 weeks, which allows to study multiple generations.
• A single mating produces a large number of offspring.
• Male and female individuals are morphologically distinct.
• Many of the hereditary variations can be studied under low power microscopes.
• About 75% of the genes causing diseases in humans are also found in Drosophila.

Key Points

• Purkinje fibres are a specialized network of cells present in the heart that help in conducting electrical impulses.
• They are specialized myocardial cells and differ from normal myocardial cells.
• These fibres help in rapid conduction of cardiac impulses along the endocardium of the ventricles.
• They lack transverse tubules and have fewer myofibrils because of their primary role in rapid conduction of impulses.
• They also contain higher amount of glycogen which can be anaerobically metabolized.
• The heart is made up of cardiac muscles capable of excitability and conductivity.
• When stimulated, these causes depolarization of electric potential called cardiac impulses, which are then conducted along the muscle fibres.

Important Points

• The conducting system of heart comprises of:
• SA Node – The sino-atrial node is also called the pacemaker as it is the first to begin the cardiac impulses and thus determine the rate of heartbeat. It lies in the right wall of the right auricle.
• AV Node – The atrio-ventricular node is also called as the pacesetter and lies in the right auricle near the junction of inter-auricular and inter-ventricular septum. It is stimulated by the contraction initiated by the SA node.
• Bundle of His – It is also called AV bundle as it arises from the AV node and come down into the inter-ventricular septum. It divides into two branches that move along the two sides of the septum. The walls of the ventricles receive the impulses through a network of specialized fibres called Purkinje fibres, which bring about synchronous contraction of the ventricles.
• The human heart is called myogenic or autorhythmic as the cardiac muscles contract on their own without any external electric impulse.

Additional Information

• Purkinje fibres are named after its discoverer Jan Evangelista Purkinje, who significantly contributed towards the field of physiology.

Key Points

• Hirudinaria is a genus of leeches that belong to the phylum Annelida.
• Botryoidal tissue is a characteristic tissue present in leeches that forms a part of its body wall.
• It is made up of vascularized cells containing a dark-brown pigment.
• It is composed of two types of cells - granular cells and endothelial-like cells.
• Botryoidal tissue is found to be secretory in function.

Additional Information

• The other layers that make up the body wall of Hirudinaria are:
• Cuticle - It is the outermost protective covering with perforations through which epidermal secretions take place.
• Epidermis - It forms the epidermal glands like the slime glands, sucker glands, prostomial glands and clitellar glands. It also secretes the mucus that covers the entire body.
• Dermis - The epidermal glands are sunken into this layer, which is composed of connective tissue.
• Muscular Layer - It comprises of various types of muscles like circular, longitudinal and oblique muscles, along with the characteristic muscle fibres having outer striated cortex or myoplasm and an inner unmodified medulla or sarcoplasm.

Key Points

• Aurelia is a genus of jelly fish that belongs to the phylum Cnidaria under under kingdom Animalia.
• It is characteristically gelatinous and umbrella-like with a circular body outline.
• The convex side is called exumbrellar surface and the concave side is called subumbrellar surface.
• The subumbrellar side has the oral arms that lead to the mouth.
• The ridges of the oral arms contain the nematocysts, which is a characteristic feature of this phylum.
• There are 4 major body axis known as perradii, along which the edges of mouth and oral arms lie.
• The 4 interradii are placed exactly midway between two perradii.
• Between each perradii and interradii there is adradii, which are 8 in number.
• Adradii are unbranched canals, while perradii and interradii are branched canals.

Important Points

• Perradii, interradii and adradii together form the radial canals which originate from the gastric pouches and join the circular or ring canal on the margin.
• The circulation of water takes place through the gastro-vascular canal system due to beating of cilia in its lining.
• The inhalant water enter through mouth into the gastric pouches and then into the circular canal through the adradial canal system.
• From the circular canal, the exhalant water returns through perradial and interradial canals into the stomach.
• From there water moves into the gullet and finally exits through the basal grooves of oral arms via gastro-oral canals.
• The separate pathways for inhalant and exhalant water prevents the mixing of water currents that enter or leave.
• This circulation of water helps in nutrition, respiration, excretion and reproduction.

Key Points

• Genes are the units of heredity, meaning they are specific sequences of nucleotides in the DNA that are responsible for a particular character trait.
• Genes are located at specific site on the chromosome.
• Each gene consists of two or more variant forms known as alleles or allelomorphs.
• These alleles are located on the same genetic locus of a chromosome and are responsible for the expression of the phenotypic trait associated with that particular gene.
• An individual inherits a pair of alleles for a gene, one from each parent.
• The alleles are mainly of two types – dominant and recessive.
• When an individual has two same types of alleles, it is called a homozygous condition. It can be homozygous dominant with two dominant alleles or homozygous recessive with two recessive alleles.
• With two dissimilar alleles it is called heterozygous condition, where one allele is dominant and the other recessive. In such cases only the dominant allele is expressed over the recessive.

Important Points

• Lethal genes or lethal alleles are the genes that lead to the death of the organism.
• They arise due to mutations in essential genes that are required for survival.
• Most of the lethal genes are expressed in recessive condition which occurs in homozygous state only.
• Lethal genes were discovered by L. Cuenot in mice for coat colour and by E. Baur in Snapdragon plants for variegated leaves.
• In mice it was shown that yellow coat colour, which is a dominant trait, was only found in heterozygotes and yellow:grey colour had a 2:1 ratio instead of 3:1. This proved that the homozygous dominant forms were lethal.
• Some recessive lethal genes have a dominant phenotypic effect but are recessive lethals. E.g., Gene for yellow coat colour in mice.
• Many lethal genes are recessive in both phenotypic as well as lethal effects. E.g., Gene producing albino seedlings in barley.

Key Points

• Ecological pyramids are graphical representations of trophic systems in an ecosystem.
• Trophic system refers to the system in which energy flows from one trophic level to another.
• Trophic level is the specific place in the food chain that is occupied by an organism based on their source of food.
• In an ecosystem, we find unidirectional flow of energy from Sun to producers to consumers.
• Ecosystems need a constant supply of energy to synthesize the molecules required in order to counteract the universal tendency of increasing disorderliness.
• In a grazing food chain, the energy trapped by the producer is passed on to the consumer or leads to death, which starts the detritus food chain and the energy is passed on to the decomposer.
• according to Lindeman's 10% Law, only 10% of the energy in a trophic level is transferred to the next, so that there can only be a limited number of trophic levels in each ecological pyramid.
• Shape of pyramid can be upright (base broader than top), inverted (top broader than base) or variable.

Important PointsEcological pyramids are mainly of 3 types:

• Pyramid of Number -
• It is based on the number of individuals in each trophic level.
• It is upright in grassland ecosystem, while inverted in parasitic ecosystem and variable in forest ecosystem.
• Pyramid of Biomass - 
• It is based on the quantitative biomass of organisms present in unit area (standing crop), for each trophic level.
• It is usually upright, but can be inverted in pond ecosystem.
• Pyramid of Energy -
• It is always upright because of the 10% Law, meaning energy keeps decreasing at successive trophic levels.

Key Points

• Starfish or sea stars belong to the phylum Echinodermata.
• Morphologically they have an oral surface and an aboral surface.
• The oral surface, facing towards the substratum, consists of:
• Mouth - It is a pentagonal aperture called actinosome angled towards the five arms. It is protected by the peristome and oral spines.
• Ambulacral grooves and spines - The mouth radiates into grooves that run into the arms and are called ambulacral grooves. These grooves are protected by the movable ambulacral spines which are calcareous in nature.
• Tube feet - or podia are a unique feature of echinoderms and are provided with suckers. They are mainly used for attachment and locomotion.
• Sense organs - The tip of each arm has a tentacle with olfactory and tactile functions and there are 5 eye-spots at the base of each tentacle.
• The aboral surface, facing upwards, consists of:
• Anus - is a minute aperture at the centre of the surface.
• Madreporite - is an asymmetrical, sieve-like plate located at the base of any two arms, which leads to the water vascular system.
• Spines - or tubercles cover the entire surface and have calcareous plates or ossicles supporting them which remain embedded in the integument.
• Papulae - or gills are tiny retractile projections from the dermal pores present between the ossicles. They are respiratory and excretory in function.
• Pedicellariae - are modified spine-like jaws that cover the entire surface.

Important Points

• The pedicellariae are used to clean the debris from the surface and keep other organisms from setting on it.
• The pedicellaria consists of a flexible stalk.
• The stalk consists of 3 calcareous plates - one basal and two jaw-like valves.
• The valves have serrations opposing each other and are articulated with the basal plate.
• Starfish may have 2 types of pedicellariae:
• Straight-type - It is a simple pedicellariae in which the valves are almost parallel to each other and attached to the base. The valves are closed with the help of 2 pairs of adductor muscles and opened with 2 pairs of abductor muscles.
• Crossed-type - The valves cross each other at the base such that the basal plate remains enclosed. They are closed and opened with 2 pairs of adductor muscles and 1 pair of abductor muscle respectively.
• Some sessile pedicellariae are also found that bear no stalk.

Mistake Points

• Please note that the answer in this solution differs from the official answer key.
• The answer has been explained in this solution.

Key Points

• The sperm is the male gametic cell that is optimized for motility.
• It can be divided into:
• Head - contains the DNA in a condensed nucleus.
• Mid-piece - contains mitochondria that gives energy for movement.
• Tail - is the whip-like flagellum that helps in propelling the sperm towards the egg.
• The tail can also be divided into the principal piece and the terminal piece.
• The tail is made of an axoneme structure and dynein motor proteins.
• The axoneme consists of 9 peripheral microtubule doublets surrounding a central pair of microtubules.
• This is known as the 9+2 configuration.
• Each of the microtubule doublets consist of a complete microtubule paired with a partially completed microtubule.
• The complete microtubule has 13 subunits or protofilaments and is called A tubule.
• The partially completed microtubule has 10-11 protofilaments and is called B tubule.
• There are radial spokes that radiate from the A tubule inwards towards the central microtubule pair.
• The dynein proteins project out from the sides of the A tubule in the form of 2 arms - outer and inner.
• Dynein are motor proteins that work in the presence of ATP.

Additional Information

• Centrioles are present in the neck region of sperm that connects the head to the tail.
• Centrioles are also made up of microtubules but with a different arrangement.
• Centrioles are made of 9 microtubule triplets on the periphery, attached by radial spokes to the central axis.
• They do not have the central pair of microtubules like in axonemes.
• Thus they have 9+0 arrangement.

Important Points

• Pollution is the unwanted change in the physical, chemical or biological aspects of the environment which is harmful to living things directly or indirectly.
• The harmful substances that cause pollution are known as pollutants.
• Pollutants are broadly classified into:
• Degradable – are the pollutants that can be broken down into simple harmless substances by natural processes, usually by microbial action. Example – Sewage, domestic wastes, agricultural wastes, paper, cloth etc.
• Non-degradable – are those that cannot be broken down by natural processes and are toxic or harmful when they accumulate. Example – Plastics, insecticides, pesticides, mercury, steel, heavy metals, synthetic fibres etc.

Additional Information

• Different types of pollution:
• Air pollution – It is caused by harmful gases like CO, CH₄, CO₂, nitrogen oxides released by burning of fuels, vehicular pollution or volcanic eruptions. These gases may produce smog, acid rain, greenhouse effect or directly cause respiratory illnesses.
• Water pollution – It is caused by various chemicals that are introduced into the water bodies by agricultural run-offs, sewage drainage, oil leakage from tankers or even direct garbage disposals. It affects aquatic life directly and other non-aquatic life forms that depend on aquatic life for food. This contaminates water, leading to various human diseases and also reduces amount of drinkable water available.
• Land pollution – It is caused by household or industrial wastes that is dumped in landfills. It may contain organic wastes, medical wastes or even hazardous industrial wastes.
• Noise pollution – It is caused by increased levels of noise in the environment due to factors like airplanes, ships, vehicles and heavy machinery. It induces stress-related problems in humans along with elevated blood pressure. It also disturbs the normal functioning of many animals by interfering with their communication or navigation systems.

Key Points

• Motility of a cell refers to its ability to move through a medium.
• This is usually achieved with the help of specialized structures of the cell like flagella.
• Flagella are hair-like structures protruding from the cell surface and has the primary function of locomotion.
• It is present in both prokaryotic and eukaryotic motile cells but differ in structure.

Important Points

• Cyanophyceae or blue-green algae are currently reclassified as cyanobacteria as they are prokaryotic in nature.
• This group of organisms is known for the absence of flagella for movement.
• Motility in some species is found to be of ‘gliding’ or ‘twitching’ movement.
• Gliding movement takes place along the direction of its long axis and involves direct contact with a surface and a mucilage secretion.
• Organisms of Cyanophyceae usually form colonies or filaments where some cells acquire the role of heterocysts for nitrogen-fixation.

Additional Information

• Phaeophyceae - have motile zoospores and gametes with two dissimilar flagella.
• Chlorophyceae - or green algae have flagellated colonial cells with mostly two flagella per cell.
• Bacillariophyceae - refer to the diatoms which were previously called golden algae. Their male gametes may have flagella.

Key Points

• Equisetum is a pteridophyte and is commonly called horsetail.
• The anatomy of the Equisetum stem is as given in the figure:
• The wavy outline shows the ridges and grooves.
• Outermost layer is epidermis which contains sunken stomata.
• Cortex is parenchymatous with patches of chlorenchyma and sclerenchyma.
• Cortex can be divided into outer and inner cortex.
• Vallecular canal is the large air canal present in the cortex below each groove.
• Then comes the endodermis that covers the vascular bundles and the pith.
• Vascular bundles are present below the ridges, alternating the vallecular canals.
• Therefore, the number of ridges and vascular bundles are equal, and similarly the number of grooves and vallecular canals are equal.
• Carinal canal is the water-filled cavity present in each vascular bundle.
• The ring of vascular bundles surround the central pith cavity.

Important Points

• Carinal canals are formed from protoxylem during elongation of internodes.
• The protoxylem disintegrates during the process of internode elongation to give rise to the carinal canals.
• They function as water-conducting channels.
• They are positioned radially opposite to the ridges(carina) and derive the name from it.
• They are arranged on the inner side of the vascular bundle.

Key Points

• Hormones refer to a group of signalling molecules present in an organism that helps in regulating various metabolic functions related to growth, physiology and behaviour.
• They are usually secreted by the endocrine glands in human body and released into the bloodstream, which then carries it to the specific target organ of the body.
• Phenols are a group of organic compounds that contain a hydroxyl group (-OH) attached to such a carbon atom that forms a part of an aromatic ring.

Important Points

• Phenols are widely used in industry for various purposes, but it is also available from natural sources.
• Phenolic natural compounds include tyrosine, serotonin and adrenaline (epinephrine).
• Adrenaline is a hormone secreted by the adrenal glands and is responsible for the ‘fight or flight’ response of our body.
• Adrenaline is derived from the amino acid tyrosine, which is also a phenolic compound.

Additional Information

• Testosterone – is the male sex hormone produced in the testis that helps in developing secondary sexual characters in males.
• Parathormone – is produced by the parathyroid gland and controls calcium concentration in serum. It stimulates the release of calcium from bones into the blood, absorption of calcium by the intestines and conservation of calcium in the kidneys.
• Prolactin – is secreted by the pituitary gland and stimulates milk production in mammals during pregnancy and after childbirth.

Key Points

• Nauplius larva refers to a larval stage of crustaceans.
• Crustaceans belong to the phylum Arthropoda and have the characteristic feature of a chitinous exoskeleton.
• As the hard exoskeleton does not allow growth, the organism undergoes repeated moulting to become an adult.
• The hard exoskeleton is shed multiple types to allow for the next developmental stage of the organism.
• Hence, there are multiple successive larval stages for a crustacean.

Important Points

• Nauplius is the first larval stage that is found in all crustaceans.
• They may sometimes hatch from the egg or pass into the egg.
• It is characterized as a free-swimming, oval-shaped larva with an unsegmented body.
• It has a large cephalothorax and an abdomen.
• There is a single median eye called the ‘naupliar eye’ present in the head region.
• There are 3 pairs of appendages:
• Antennules – are the first pair of appendages and is un-jointed.
• Antennae – are the second pair of appendages that help in swimming and are biramous, meaning they divide to form two branches.
• Mandibles – are the last pair of appendages, are biramous and also help in swimming.

Additional Information

The various other crustacean larval stages are:

• Metanauplius
• Cypris
• Protozoea
• Zoea
• Metazoea
• Mysis
• Megalopa
• Alima
• Phyllosoma

Mistake Points

• In these types of questions, we need to assess the given statements first and then choose the correct/incorrect (whichever is asked in the question) combination of the statements from the given options.

Key Points

• Statement 1- Saliva acts as a lubricant: TRUE
• One of the primary functions of saliva is to lubricate the food as well as the mouth (buccal cavity).
• Saliva consists of mucus that helps in masticating the food in the buccal cavity and then binding the masticated food into a bolus.
• The bolus is lubricated by saliva so that it can easily slip through the oesophagus without damaging the mucosal layer.
• Statement 2- Salivary amylase converts starch into glucose: FALSE
• Starch is a complex carbohydrate formed as a polymer of glucose molecules.
• Saliva contains the enzyme amylase that acts on starch to cleave the polymer into smaller carbohydrates.
• Amylase acts on starch to form dextrin, which is then broken down into disaccharides like maltose.
• Glucose is generated from maltose by the action of a different enzyme called maltase.
• So, we can say that salivary amylase converts starch to maltose, but not glucose.
• Statement 3- The pH of saliva is about 3.4: FALSE
• The pH of saliva is near neutral.
• It ranges from 6.2-7.6 and the average normal pH is 6.7.
• The pH is maintained by eliminating the carbohydrates which can be metabolized by bacteria and also the acids formed by bacterial activity.
• Statement 4- Chloride ions are essential for the activity of salivary amylase: TRUE
• Chloride ions help in increasing the reactivity of the enzyme amylase.
• Transport of chloride ions across the epithelial membrane also drives saliva secretion process.

Additional Information

• Some other functions of saliva:
• Removing food debris from buccal cavity and preventing dental plaques.
• Protecting against teeth demineralization.
• Lubricating teeth to prevent dental wear.
• Preventing infections by antimicrobial activity of lysozyme.
• Solubilizing the food so that taste stimuli are carried to the taste receptors.
• It also starts the anticipatory phase of digestion that prepares the body to metabolize the ingested food efficiently.

Key Points

• Pila is a genus of snails belonging to the phylum Mollusca.
• They are freshwater snails that can also live on the land and hence are called amphibious.
• In order to get oxygen from water as well as from air when on land, they require a double mode of respiration.
• They have gills for aquatic respiration and pulmonary sac for aerial respiration.
• They come out of water to respire when the oxygen concentration in water decreases.

Important Points

Aquatic respiration by gills:

• The ctenidium is present in the right chamber of the mantle cavity or branchial cavity.
• The gill is of monopectinate type, meaning the triangular lamellae are arranged parallelly in a single row along the central axis of the gill.
• The basal ends of the lamellae are attached to the mantle wall epithelium, while the other ends hang free in the mantle cavity.
• The size of the lamellae gradually increases towards the centre.
• The oxygen-carrying water current moves into the mantle through the left siphon.
• As the water flows over the gills, exchange of gases take place with the blood vessels of the gills.
• The water is then expelled out of the mantle cavity through the right siphon.
• The respiratory siphons are formed by fleshy projections over the feet called nuchal lobes.

Aerial respiration by pulmonary sac:

• Osphradium is the comb-like organ that helps them to estimate oxygen concentration in water.
• The pulmonary sac is present in the left chamber of the mantle cavity, which is also called pulmonary cavity.
• The pneumostome or the opening to the pulmonary chamber is guarded by two valves.
• Aerial respiration while in water:
• While still in the water, it can come to the surface to respire.
• The left nuchal lobe is expanded and rolled up to form the respiratory tube.
• The end of the tube moves above the water surface to suck in air from atmosphere.
• The other end comes into the pneumostome of the pulmonary chamber.
• After the gaseous exchange, the expelled air moves out through the respiratory tube.
• During this respiration process, the branchial chamber remains separated from the pulmonary chamber.
• Aerial respiration when on land:
• They come to land during dry seasons, when the water levels are very low.
• During this time, the entire pulmonary sac fills up with atmospheric air.
• They do not use the siphons or respiratory tube.

Additional Information

• Pila is found mostly in the tropical and sub-tropical habitats, which have extreme seasonal variations
• They are adapted for aestivation (summer-sleep) during dry, summer season.
• During aestivation, they respire minimally with the air stored in the pulmonary sac.
• The shell remains completely closed by the operculum.
• The respiratory pigment is haemocyanin.

Key Points

• Bioassay refers to the assessment methods that utilize living organisms to detect the presence and potential toxicity of chemical substances.
• These methods are often used to screen potential hazardous chemicals in contaminated soil, water and food.
• It helps us to determine the level of pollution at a certain place.
• It also shows that even essential micronutrients show toxicity when present in higher concentrations.
• Microbial organisms like bacteria and fungi can be used in bioassays.
• In bioassay methods, the effect of the toxicity on the living organism is studied.

Important Points

• Several fungi are often used to detect metal ions from a medium.
• One such example is Aspergillus niger that helps in detection of copper in trace amount.
• Several experiments have shown that copper ions are taken up by the fungal cells and accumulated in the cytoplasm.
• This results in cytotoxicity of the fungal cell and reduces the pathogenicity of the fungus.
• This implies that copper ions can used in antifungal treatment therapies.
• This is also supported by the ancient tradition of keeping water in copper vessels, which was believed to be healthier for consumption.
• This has been scientifically proved as copper contact surfaces and nanoparticles kill the fungal spores of various species.
• It can also result in DNA fragmentation and cell membrane damage that leads to cell lysis.
• Metal ions can also bind to enzymes and proteins, causing cell damage in microbes.
• These properties can be utilized in pharmacological purposes.

Key Points

• Geological time refers to that period of Earth's history which can be studied geologically from the rocks.
• Geological time scale is how that timeframe is sub-divided like a calendar for our understanding.
• The fossils that are found in the rock strata helps in establishing the timeline for appearance or disappearance of species.
• It also helps us in studying the timelines of biological evolution of different species.
• It is divided into eons, eras, periods and epochs.

Important Points

• Angiosperms are the flowering plants that evolved relatively recent on the geological time scale.
• Among the angiosperms, the dicots originated first and then the monocots, which have more advanced evolutionary features.
• The fossil records show that the earliest appearance of the angiosperms happen during the Cretaceous period, which is about 140-65 million years ago and comes in the Mesozoic era.
• But the angiosperms developed and diversified during the Coenozoic era.
• The earliest angiosperms were more herbaceous similar to the present-day families like Ranunculaceae.
• Later on these gave way to the woody plants that led to the formation of forests and grasslands.
• The development of flowers in angiosperms required pollination to take place in order to avoid self-pollination and fertilization.
• This led to the co-evolution of insects and other organisms that would help in pollinating the angiosperms.

Key Points

• The mammalian kidney consists of numerous tubular structures called nephrons.
• Each nephron has 2 parts:
• Glomerulus - is a tuft of capillaries that takes part in the first step of urine formation, i.e. glomerular filtration.
• Renal Tubule - starts with the Bowman's capsule, continues as proximal convoluted tubule(PCT), Henle's loop and the distal convoluted tubule(DCT).
• The glomerulus together with Bowman's capsule is called malpighian or renal corpuscle.
• Glomerulus:
• It helps in filtration of blood through 3 layers of cells - endothelium of glomerular blood vessels, epithelium of Bowman's capsule and the basement membrane between the two layers.
• The epithelial cells of Bowman's capsule are called podocytes.
• Podocytes are intricately arranged such that there are minute pores in between, which are called filtration slits or slit pores.
• Proximal Convoluted Tubule:
• It helps in maintaining the pH and ionic balance of the body fluids.
• ​Loop of Henle:
• The loop of Henle has two limbs - the descending limb and the ascending limb.
• As the glomerular filtrate moves in opposite directions along the two limbs of Henle's Loop, it gives rise to a counter-current mechanism, which is responsible for concentrating the urine.
• Minimum reabsorption takes place.
• It plays important role in maintenance of high osmolarity of medullary interstitial fluid.
• It helps in concentrating the urine so that water is conserved in the body.
• Distal Convoluted Tubule:
• There is conditional reabsorption of water and Na+.
• It helps in maintaining the pH and sodium-potassium balance in blood.

Important Points

Urine formation involves 3 major processes:

• Glomerular Filtration - The glomerular capillary blood pressure causes the filtration of blood through the 3 layers. It is also called ultrafiltration because blood is finely filtered through the podocytes such that all plasma contents except proteins pass onto the lumen of Bowman's capsule.
• Reabsorption - The GFR is about 4.5 times that of the urine output, suggesting that almost 99% of the filtrate is reabsorbed by the renal tubules by active or passive mechanisms.
• PCT - actively reabsorbs all of glucose, 75% of amino acids & Vitamin C, 90% of bicarbonate (HCO₃^-), 70% of Na⁺, 75% of K⁺ and huge amount of Ca²⁺ from the glomerular filtrate. This makes for the reabsorption of almost 80% of the total glomerular filtrate.
• Ascending Limb of Henle's Loop - actively reabsorbs about 25% of K⁺ and some Cl^-, while some Na⁺ is reabsorbed by diffusion.
• DCT & Collecting Tubules - reabsorb some Na⁺ from filtrate in exchange of K⁺ from interstitial fluid.
• Secretion - The tubular cells secrete substances like H⁺, K⁺ and ammonia into the filtrate. It is an important step because it helps in maintaining ionic balance of body fluids.

Additional Information

• Renal plasma flow - is the amount of plasma (blood) that passes through the glomerulus of all the nephrons in both the kidneys per minute.
• Cardiac output - is the amount of blood pumped by the heart in a minute.

Cardiac output = Stroke volume (Blood pumped by each ventricle per minute) × Heart Rate

• About 1000-1200ml of blood or 650ml of blood plasma is the renal plasma flow, which is one-fifth of the cardiac output.
• Glomerular Filtration Rate (GFR) - is the amount of blood filtered out of the glomerulus and is clinically used to diagnose kidney diseases.
• The GFR for a healthy individual is about 125ml/min or 180L per day.
• Urine output - is the amount of urine excreted from the body per day and is about 1.5L on an average.

Key Points

• The scientific name of house fly is Musca domestica and it belongs to the phylum Arthropoda.
• Houseflies are general feeders, meaning they feed on any organic matter, starting from food items to fecal matter.
• They typically have sponging mouth parts, meaning they absorb the food particles in liquid form like a sponge.
• In order for the sponging mouth parts to work, the solid foods are liquefied by saliva before uptake.
• These organisms cannot bite or chew their food as they have lost their maxillae and mandibles which are essential for the process.

Important Points

• The mouthparts include a proboscis, epipharynx, hypopharynx, labium and a pair of maxillary palps on the side of the proboscis.
• The proboscis is divided into:

1. Rostrum -

• It is the basal part and encloses the pharynx and salivary duct.
• Though maxillae are absent, the maxillary palps are present on the rostrum.

2. Haustellum -

• It is the middle part of proboscis and proximal part of labium, bearing a median groove on the dorsal side.
• It encloses the food canal formed by the labrum-epipharynx and the salivary canal of hypopharynx.

3. Labellum -

• It is the terminal part of the proboscis that is made up of 2 lobes called labella.
• The preoral opening is present between the two labella.
• It also comprises of grooves covered by semicircular chitinous rings that make them look like a trachea and hence called pseudotrachea.
• All pseudotracheae converge into the preoral opening to take in the liquefied food.
• The haustellum and labellum are modified labium.
• The mechanism of food intake involves the haustellum and labella being thrust out from underneath the rostrum such that the labella get pressed against the food.
• Saliva is released from the labella onto the food to dissolve or liquefy it.
• This liquefied food then enters the pseudotracheae by the capillary action and then pass through the food channel into the oesophagus.

Additional Information

• Houseflies are responsible for transmitting a number of diseases and are often indicative of unhygienic conditions.
• Mosquitoes are also common carriers of human diseases like malaria, dengue and yellow fever.
• Cockroach is common in most households and buildings and spread a lot of bacterial diseases.
• Honey-bee is a beneficial insect for humans because they help in pollinating flowers and can be commercially grown for honey.

Key Points

• Central dogma of molecular biology states that flow of genetic information takes place from DNA to RNA to proteins.
• Transcription of DNA to RNA is based on the complementarity of their strands.
• However, no such complementarity exists between the RNA and the proteins for translation to take place.
• Evidences show that changes in nucleic acids also caused changes in the amino acids of the proteins.
• This suggested the existence of a Genetic Code that directs the synthesis of amino acids that form the proteins.
• It was George Gamow who suggested that if the 4 nitrogen bases of nucleic acids have to code for the 20 non-essential amino acids produced in the body, the code should constitute a combination of bases.
• The nitrogen bases include Adenine (A), Guanine (G), Cytosine (C) and Uracil (U) for an RNA.
• A permutation combination of 2 bases would give: 4² = 16 amino acids only. Thus, it has to be a combination of 3 bases giving: 4³ = 64 codons.
• Thus it was concluded that 64 codons would code for the 20 amino acids with some codons being signal codons too.

Important Points

• The salient features of the genetic code are:
• Triplet codon - Each codon is a triplet of any 3 bases. 61 of the codons code for amino acids and 3 codons act as STOP codons.
• Start codon - The codon AUG acts as a START codon or Initiator codon as well as codes for Methionine(Met).
• Unambiguous & Specific - One codon codes for only one amino acid.
• Degenerate - Some amino acids are coded by more than one codon.
• Contiguous - The codon in mRNA is read in a continuous manner without any punctuation.
• Universal - A codon codes for a specific amino acid for all organisms. Exception - Some mitochondrial codons and some protozoans.
• The 3 stop codons are:
• UAG - Amber
• UGA - Opal
• UAA - Ochre

Key Points

• Pollution is the unwanted change in the physical, chemical or biological aspects of the environment which is harmful to living things directly or indirectly.
• The harmful substances that cause pollution are known as pollutants.
• Pollutants can be of two types based on their source:
• Primary Pollutants - are the pollutants that come directly from a source. Example - Carbon dioxide, Carbon monoxide, oxides of nitrogen, sulphur dioxide.
• Secondary Pollutants - are the pollutants that are not directly emitted from the source but are caused by other primary pollutants. Example - Photochemical oxidants like ozone, sulphur trioxides.
• Ground-level ozone or tropospheric ozone is formed when hydrocarbons combine with nitrogen oxides in the presence of sunlight.
• Atmospheric sulphur trioxide (SO₃) is an intermediate which is formed when sulphur dioxide (SO₂) reacts with water droplets in the air to form sulphuric acid. And this in turn causes acid rain.

Mistake Points

• Ozone is not a pollutant when present in the stratosphere because it is formed naturally to protect us from the UV radiation.
• Ozone in the troposphere or near ground-level is a secondary pollutant and is a major cause of urban smog.
• Sulphur dioxide is a primary pollutant but sulphur trioxide (SO₃) is a secondary pollutant that causes acid rain.

Additional Information

Different types of pollution:

• Air pollution -
• It is caused by harmful gases like CO, CH₄, CO₂, nitrogen oxides released by burning of fuels, vehicular pollution or volcanic eruptions.
• These gases may produce smog, acid rain, greenhouse effect or directly cause respiratory illnesses.
• Water pollution -
• It is caused by various chemicals that are introduced into the water bodies by agricultural run-offs, sewage drainage, oil leakage from tankers or even direct garbage disposals.
• It affects aquatic life directly and other non-aquatic life forms that depend on aquatic life for food.
• This contaminates water, leading to various human diseases and also reduces amount of drinkable water available.
• Land pollution -
• It is caused by household or industrial wastes that is dumped in landfills.
• It may contain organic wastes, medical wastes or even hazardous industrial wastes.
• Noise pollution -
• It is caused by increased levels of noise in the environment due to factors like airplanes, ships, vehicles and heavy machinery.
• It induces stress-related problems in humans along with elevated blood pressure.
• It also disturbs the normal functioning of many animals by interfering with their communication or navigation systems.

Key Points

• Plantago ovata is a medicinal plant that belongs to the family Plantaginaceae.
• It is an annual herb that is widely grown in India.
• Isaphgol is the common name for the plant as well as their seeds and husks that are used in dried forms or decoctions.
• It is traditionally used for various medicinal purposes.
• The most common use of Isaphgol is as a laxative for constipation and for chronic diarrhea.
• It has the following features:
• Colour - Light Brownish.
• Odour - Faint to none.
• Taste - Bland, mucilaginous.
• Major Constituents - Hydrocolloids as mucilage.
• Pharmacological properties - Demulcent, anti-diarrheal, anti-constipation, hypocholestrolemic and hypoglycemic.
• Uses - For medicinal purposes, as laxative, gelling agent and as stabilizer in ice-cream industry.

Additional Information

• Strychnos nux-vomica - is a tree whose seeds contain 'strychnine' which was previously used for medicinal purposes, but is now used as pesticide.
• Erythroxylum coca - The leaves of this plant are the source of the drug 'cocaine'.
• Digitalis purpurea - or fox-glove is also a plant of Plantaginaceae family and is the source of the cardiac glycoside 'digitalis', which is used to treat heart rhythms.

Key Points

• Marchantia is a bryophyte which is commonly known as liverwort.
• It is a heterothallic plant with the antheridiophore and archegoniophore on different thalli.
• The antheridiophore and archegoniophore bear the antheridia and archegonia respectively.
• The archegonium is a flask-shaped structure with a swollen venter near the base and an elongated neck.
• The venter is surrounded by jacket cells and contains a large egg cell and venter canal cell.
• The neck consists of neck canal cells enclosed by the neck cells.
• Archegonia are placed upright on the archegoniophore for fertilization to take place.
• After fertilization the archegonia is reversed from acropetal to basipetal arrangement such that a thick tissue called perichaetium flanks the archegonial row.

Important Points

• The zygote develops to form the sporophyte which is differentiated into:
• Foot - It is the basal swollen part that helps in anchoring and gives nutrition to the sporophyte.
• Seta - It is the stalk that connects the foot to the capsule.
• Capsule - It is an enlarged oval structure with a single jacket layer, which encloses the sporogenous tissue in young sporophytes.
• The sporogenous tissue differentiates into:
• Spore mother cells - undergo meiosis to form the haploid spore tetrad.
• Elater mother cells - give rise to diploid and sterile elaters.
• During sporophyte development, the cells of venter wall gives rise to the calyptra.
• Calyptra is a 2-3 layered covering that protects the developing sporophyte.
• Perigynium or pseudoperianth is a collar-like outgrowth outside calyptra that arises from the basal part of archegonium.
• Towards maturity of sporophyte, the seta elongates to push out the capsule through the calyptra, perigynium and perichaetium.

Key Points

• Water potential is the potential energy of water in a given system and is denoted by (Ψ).

      Ψ_water = Ψ_s + Ψ_p + Ψ_g + Ψ_m

where, Ψ_s = Solute potential

Ψ_p = Pressure potential

Ψ_g = Gravity potential

Ψ_m = Matric potential

• All the components given above influence the water potential of a system.

Important Points

• Solute potential represents the component of water potential that is due to the presence of solute molecules.
• Water tends to move from a system of higher water potential (hypotonic solution) to that of lower water potential (hypertonic solution).
• Solute potential or osmotic potential is the potential of water molecules to move from a hypotonic solution to a hypertonic solution across a semi-permeable membrane.
• It always has negative value because solutes lower the water potential of the system.
• Solutes get dissolved in water by binding to water via hydrogen bonds.
• Some of the energy is tied to the H-bonds between water and solute molecules and are not available to work in the system.
• Hence, the solute potential is reduced, which eventually also reduces the water potential.
• As pure water has no solute molecule, its solute potential is zero and for every other solution it is negative.

Additional Information

• Solute potential plays an important role in maintaining the soil-plant-atmosphere continuum.
• This refers to the pathway by which water moves from soil through plant cells and transpired from leaves.
• This movement of water depends on the solute potential of soil water (Ψ^soil), root water (Ψ^root), stem water (Ψ^stem), leaf water (Ψ^leaf) and water in the atmosphere (Ψ^atmosphere).
• The condition that drives transpiration in plants is:

Ψ^soil > Ψ^root > Ψ^stem > Ψ^leaf > Ψ^atmosphere

• Water moves from soil to plant roots via osmosis and hence, solute potential is also called osmotic potential.

Key Points

• Growth in plants takes place in two ways:
• Primary growth - It is the main form of growth in a plant that mostly occurs due to activity of apical meristems.
• Secondary growth - This is found in most dicot plants exhibiting an increase in girth.
• Secondary growth is caused by 2 lateral meristems:
• Vascular Cambium - It is responsible for forming secondary xylem and secondary phloem.
• Cork Cambium - It forms the periderm (bark) towards the outer periphery in older plants.
• In dicot stems, a continuous cambial ring is formed by the intrafascicular and interfascicular cambium.
• The cambial ring becomes active and starts to cut off cells on both sides of the ring to produce:
• Secondary Xylem - It is formed towards the inner side as a compact mass due to the higher activity of cambium on the inner side.
• Secondary Phloem - It is formed towards the periphery and gradually gets crushed due to increased formation of secondary xylem.

Important Points

• The secondary xylem is distinguished into 2 forms:
• Heartwood - It is the greater part of secondary xylem formed towards the inner side.
• Sapwood - It is formed from secondary xylem towards the periphery and is located between the bark and heartwood.

Mistake Points

• Please note that the official answer key shows option 1 as the correct answer.
• But it has been changed to option 4 here because Cetraria does not produce orchil dye.
• Also, Cetraria lichen gives a yellow-coloured dye.

Key Points

• Orchil or Archil is a blue-violet coloured dye that is obtained from some lichens by fermentation.
• The lichens that yield this dye are called orchil lichens.
• Examples of orchil lichens:
• Roccella tinctoria - It is the most commonly used orchil lichen.
• Evernia prunastris - It is a lesser known lichen that is used to obtain orchil dye.

Important Points

• Evernia prunastri is a fruticose lichen which is also known as oak moss.
• It grows in the mountains of the Northern hemisphere.
• It is a sage-coloured lichen that gives a bluish-purple dye when left to soak in water and ammonia.
• It has been used since ancient times for dyeing wool and silk fabrics.

Additional Information

• Cetraria islandica -
• It is also known as Iceland moss.
• It has an upright moss-like growth habit.
• It is used as food supplement for cattle and sheep.
• It yields a yellow-brown dye and also contains the lichen starch called lichenin.
• Ramalina fastigiata -
• It is a fruticose lichen which is commonly found on tree trunks or branches.
• It is used for biomonitoring air pollution.
• Cladonia rangifera -
• ​It is commonly called reindeer moss or lichen.
• Reindeer moss is so called because it is a source of food for reindeer during winter in Scandinavian countries.

Important Points

• Aestivation is the mode of arrangement of petals in a floral bud with respect to the other petals of the same flower.
• Aestivation can also refer to the arrangement of sepals in a floral bud, which can be different from the aestivation of petals.

Key Points

• Aestivation is of 4 major types:
• Valvate - The petals of a whorl touch each other at the margins without overlapping. Example - Calotropis.
• Twisted - The margin of one petal overlaps with the margin of the next and so on. Example - China rose.
• Imbricate - The margins of the petals overlap each other but in a random order. Example - Cassia.
• Vexillary - This is also called papilionaceous type and consists of 5 petals of different shapes and sizes. The largest petal (called standard) overlaps 2 lateral petals (wings), which in turn overlap the other 2 smallest anterior petals (keel). Example - Pea.

Additional Information

• Pea or Pisum sativum is a leguminous plant that belongs to the family Fabaceae.
• Some of the reproductive features of P. sativum are:
• Inflorescence - Racemose.
• Flower - Bisexual and zygomorphic.
• Calyx - 5 Sepals, gamosepalous with imbricate aestivation.
• Corolla - 5 petals, polypetalous with vexillary aestivation.
• Androecium - 10 stamens, diadelphous; anther dithecous.
• Gynoecium - Monocarpellary, superior unilocular ovary.
• Fruit - Legume.
• Seed - Non-endospermic.

Key Points

• A greenhouse is a glass building that provides optimum temperature for plants to grow in cold climates. The glass traps the heat inside to keep it warm.
• Carbon dioxide acts in a very similar way for our atmosphere in trapping the heat and thus known as a greenhouse gas.
• The CO₂ present in the atmosphere absorbs the infrared radiation and re-emits the heat back to the surface.
• 50% of the heat is lost to space and rest 50% comes back to the Earth's surface.
• This keeps the average temperature of Earth inhabitable.
• This phenomenon is called greenhouse effect.

Important Points

• The primary greenhouse gases include water vapour, carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O).
• Various human activities like burning of fossil fuels has led to a significant increase of greenhouse gases in the atmosphere over the past two centuries.
• This has in turn led to the global warming which is a global concern for life on Earth.
• Global warming causes increase in global average temperature, melting of polar ice, increase of sea-level, etc.

Additional Information

• SO₂ - Sulphur dioxide is a toxic gas produced by burning of sulphur-containing materials and naturally from volcanic eruptions.
• NO₂ - Nitrogen dioxide is also a by-product of burning of fuels and also production of fertilizers.
• O₃ - Ozone present in the atmosphere protects us from UV radiation by absorbing it. It can also act as a secondary greenhouse gas.

Key Points 

• The 5-kingdom classification of Whittaker places the organisms under Monera, Protista, Fungi, Plantae and Animalia.
• Kingdom Animalia is further divided into 11 phyla, which are in order of evolution, meaning they are placed from the most primitive to the most advanced.
• The phyla are: 
• Porifera ⇒ Coelenterata (Cnidaria) ⇒ Ctenophora ⇒ Platyhelminthes ⇒ Aschelminthes ⇒ Annelida ⇒ Arthropoda ⇒ Mollusca ⇒ Echinodermata ⇒ Hemichordata ⇒ Chordata.

Important Points

• A connecting link is such an organism which has the transitional characters of two taxonomical groups between which it forms the link.
• Neopilina is a modern monoplacophoran genus that is considered to be a connecting link between the phyla Annelida and Mollusca.
• It is placed under the phylum Mollusca and is the most primitive species of this phylum.
• It is named as 'new Pilina', after the extinct monoplacophore species Pilina that lived about 400 million years ago.
• Neopilina shows features from Annelida as well as Mollusca.
• It has trochophore larva like Annelida.
• It shows the presence of a flat muscular foot like in Mollusca.

Additional Information

Key Points

• Transcription is the process of copying the genetic information from one strand of DNA onto the RNA.
• It takes place with the help of the enzyme RNA polymerase (RNA pol).
• It takes place at a defined region of DNA that is known as the transcription unit.
• A transcriptional unit can be recognized by the following:
• Template strand - It acts as the template for RNA pol to act on and has 3'→5' polarity.
• Coding strand - It has 5'→3' polarity and is used as a reference strand for denoting upstream or downstream of a gene.
• Structural gene - It contains the nucleotide sequence that actually codes for the required RNA.
• Promoter - It is the nucleotide sequence that acts as a binding site for RNA pol enzyme.
• Terminator - It is the nucleotide sequence that defines the end of transcription by binding the Rho (ρ) factor.
• The promoter is located upstream of the gene, while the terminator is located downstream of the gene.
• Upstream and downstream indicate towards the 5'-end and the 3'-end of coding strand respectively.

Important Points

Basic steps of prokaryotic transcription:

• Initiation -
• RNA pol enzyme binds at the promoter site of DNA with the help of sigma (σ) factor and initiates transcription.
• Elongation -
• The sigma factor is released and RNA pol moves along the DNA to untwist the DNA double helix.
• RNA pol uses nucleoside triphosphates for polymerization of RNA that remains bound to the DNA in the transcription bubble.
• Some of the newly synthesized RNA is released from the enzyme as a single strand.
• Only a part of the RNA remains bound as DNA-RNA hybrid.
• Termination -
• It is carried out by the Rho protein that binds to the terminator site.
• When the Rho protein comes across the RNA pol enzyme, the RNA is unwound from the DNA.
• The nascent RNA is released along with the enzyme.
• The DNA double helix reforms and Rho protein dissociates, terminating the transcription process.

Key Points

• Ecology studies the interactions of organisms between each other and with the environment.
• The environment faces seasonal and annual variations that helps in forming biomes.
• The localized variations in a biome further leads to the formation of different habitats.
• The habitat comprises of various abiotic and biotic components that also act as factors for causing the variations.
• The abiotic factors include - temperature, water, light and soil.
• The biotic factors include the organisms present in that habitat and their interactions.

Important Points

• Temperature is an important abiotic component because it affects the enzyme kinetics in organisms and hence regulate various metabolic activities.
• Organisms are divided into 2 groups based on temperature component:
• Eurythermal - Organisms that can tolerate and thrive in a wide range of temperatures. E.g. - Dog, Cat, Man.
• Stenothermal - Organisms that are restricted to a narrow range of temperatures. E.g. - Penguin, Crocodile.
• Homeostasis is the regulation of internal environment relative to the external environment such that the physiological processes can continue at optimum and constant rate.
• Organisms can also be categorized according to their response towards such abiotic factors like temperature.
• Homeothermal - These are the warm-blooded organisms that maintain their body temperature at an constant optimum range at which physiological processes can continue even when the environment changes. For example, when external temperature is more than internal temperature, humans sweat to decrease the body temperature by evaporative cooling. On the other hand, when external temperature is less than body temperature, we shiver to generate heat and thus increase our internal temperature.
• Poikilothermal - These are the cold-blooded organisms that cannot maintain homeostasis and their body temperature changes with environment temperature. Example - All reptiles.

Additional Information

• Water is also an important abiotic factor affecting organisms with its pH and chemical composition.
• Water bodies mainly differ in their salinity levels.
• Organisms are divided into:
• Euryhaline - Organisms that can tolerate a wide range of salinities. E.g. - Molly fish.
• Stenohaline - Organisms that are restricted to a narrow range of salinities. E.g. - Goldfish.

Key Points

• Plant cells have cell walls around their plasma membranes for rigidity and support.
• Plant cell walls have plasmodesmata through which intercellular connections are maintained.
• Plasmodesmata are cell junctions in plants which form fine cytoplasmic channels between cells.
• These channels are about 20-40 nm in diameter.
• Desmotubule is a narrow cylindrical structure that runs through the centre of the plasmodesmata.
• Desmotubules are continuous with the endoplasmic reticula of adjoining cells.
• There is a cytosolic annulus between the inner wall of plasmodesmata and desmotubule through which molecules can pass.

Additional Information

• These plasmodesmata connections allow free movement of small metabolites and growth hormones between plant cells.
• They help in intercellular transport of RNA and proteins.
• They have a dynamic structure to selectively allow transport of some macromolecules.
• These are very important in the movement of proteins from companion cells to sieve elements of the phloem.
• The symplast pathway involves movement of molecules through the plasmodesmata.
• The symplast pathway is vital for the cells with casparian strips.

Key Points

• Ecosystem is defined as a functional system comprising organisms of a given area interacting with the physical environment and also among themselves.
• This interaction allows flow of energy, leading to a clearly defined trophic structure, biodiversity and nutrient cycles.
• The various components of an ecosystem can be broadly divided into:
• Abiotic components - include the non-living components of the environment present in that ecosystem. They can be climatic conditions as well as chemical substances present in the environment.
• Biotic components - include all the living forms in that ecosystem. They can be autotrophic or heterotrophic in their mode of nutrition.

Important Points

• Ecosystems can be broadly classified into 3 types:
• Natural - These ecosystems are formed gradually due to natural forces in the environment. They are usually complete with producers, consumers and decomposers. It is the most commonly found ecosystem.
• Artificial - They are man-made ecosystems where the components are introduced and controlled by us as per our need. Example - Crop fields, aquarium.
• Incomplete - This is a special type of ecosystem where producers are absent. Example - Caves and depths of the ocean.
• Natural ecosystems are further classified into:
• Terrestrial - This includes all land ecosystems like grasslands, deserts and forests.
• Aquatic - This includes marine ecosystems like sea and ocean. It also includes freshwater ecosystems like ponds, lakes and rivers.

Additional Information

• Succession -
• Ecological succession is the gradual change in the species composition of a given area caused due to successive colonization of different plant communities.
• Every ecosystem we see is actually in one or the other succession stages called seral stages.
• Whether it starts from a dry (xeric) area or from a wet (hydric) area, succession always leads to a forest community.
• The species that first colonizes an area is called pioneer species and the last stage of succession is called climax community, which shows great biodiversity.
• Ecotone -
• It is the transitional area between two different ecosystems caused due to sudden changes in the environment.
• For example, mangroves are an ecotone between terrestrial and marine ecosystems.
• They include species that are highly adaptive to colonize the transitional area.
• They may also include species that are common to both the ecosystems surrounding the ecotone.
• Community -
• An ecological community is defined as an assemblage of two or more species populations in a particular area.
• These communities interact with the environment, which keeps constantly changing.
• As a result, the environment causes change in the communities as well as gets modified by the communities.
• This interaction is the basis for ecological succession.

Key Points

• Fasciola is a parasite that belongs to the phylum Platyhelminthes under Animalia kingdom.
• It is commonly known as liver fluke as it infects the livers of mammals including humans.
• The life cycle of Fasciola involves a primary host (mammals) and an intermediate host (molluscs).
• They may form 7 different development stages: egg, miracidium, sporocyst, redia, cercaria, metacercaria and adult fluke.
• The larval stages in sequence are:

Miracidium ⇒ Sporocyst ⇒ Redia ⇒ Cercaria ⇒ Metacercaria​

Important Points

• Miracidium -
• It is the first larval stage that comes from the fertilized egg.
• It is covered by cilia and epidermal plates.
• Internally it contains an apical gland, a pair of penetration glands, 2 eye spots, 2 flame cells, a rudimentary gut and several germ cells.
• It infects the mollusc host (snail) and loses most of its body parts.
• The germ cells grow and divide to form germ balls inside the host.
• Sporocyst -
• It is the second larval stage that develops from miracidium inside the host.
• It is a non-feeding stage that depends on the host for nutrition.
• It is a sac-like structure covered with cuticle.
• One sporocyst gives rise to 5-6 redia.
• Redia -
• It comes out of the sporocyst by rupturing the sporocyst wall.
• The germ cells give rise to a number of daughter redia that develops into cercaria.
• Cercaria -
• It is a free-living stage produced from redia.
• It is considered as a young fluke of sexual generation.
• Only rudiments of reproductive germ cells remain.
• It contains cystogenous glands to form a cyst for the future larva.
• Metacercaria -
• It is the last larval stage and is infective for the final host (sheep).
• It is the juvenile form of Fasciola.
• It is formed by the encystation of cercaria.
• Thus, one cercaria larva produces only one metacercaria larva.

Key Points

• Laccifer lacca is commonly called the Indian lac insect and belongs to the phylum Arthropoda.
• It is found in tropical and subtropical regions and widely exploited in India.
• It is a type of scale insect, who have a hard covering around their body that is formed by their own secretions.
• The females usually produce the bulk of the resin that forms a coating around the twig of dwelling.
• The insects live as parasites, feeding on the plant sap.
• Shellac or lac is the resin secreted by these insects by certain abdominal glands.
• The secretion starts after settling on a twig and starts hardening around the twig.
• Some of the features of Shellac include:
• Colour - Amber to reddish.
• Texture - Hard, brittle, resinous.
• Odour - Odourless when cold, characteristic smell on heating.
• Pharmaceutical use - As a coating for medicine tablets.
• Uses - As ingredient for colours, abrasives, sealing wax, glazes, nail polish, hair spray and furniture polish.

Additional Information

• Apis indica - is the Indian honey bee, which is widely cultivated (apiculture) to produce honey and bee-wax.
• Bombyx mori - is the domesticated silk moth that is used in silk production or sericulture.
• Dactylopius coccus - is a scale insect that produces carminic acid, from which we derive the carmine red dye.

Key Points

• Amphioxus or lancelet belongs to the sub-phylum Cephalochordata.
• They are marine animals with slender, fish-like bodies.
• They have some distinct features:
• Notochord extends from head to tail.
• Notochord is persistent throughout life.
• Nerve cord is dorsal and hollow.
• They live in shallow waters and are ciliary feeders.
• They depend on planktonic microorganisms that enter through a water current into the mouth.

Important Points

• The mouth leads to the space called buccal cavity or vestibule, which is enclosed by oral hood.
• The margins of oral hood is lined by oral cirri bearing sensory papillae.
• The oral cirri remain converged inward such that it forms a sieve to prevent entry of large particles into vestibule.
• The main parts of the buccal cavity include:
• Wheel Organ - or Muller's organ represents 6-8 pairs of finger-like folds of oral hood epithelium with ciliated grooves and ridges in each.
• Velum - It is the circular, membranous structure at the posterior end of buccal cavity with a central enterostome leading into the pharynx.
• The velum has velar tentacles that act as sphincters to open or close the mouth.
• The mid-dorsal groove of wheel organ is called Hatschek's groove that ends in a depression called Hatschek's pit in the roof of buccal cavity.
• The wheel organ produces a vortex of water to put food particles into the mouth.
• Some leftover food particles get mixed with the mucus secreted by the Hatschek's groove to pass it to the pharynx.

Key Points

• Casuarina is an evergreen tree that belongs to the family Casuarinaceae.
• Although it resembles conifers in having pine-like needles. but it is actually an angiosperm and thus undergo double fertilization.
• Double fertilization is an unique reproductive process in angiosperms where two fertilization processes occur for each embryo.
• The reproduction process is divided into 3 events:
• Pre-fertilization - It includes elaborative processes like gametogenesis in male and female organs, pollination and pollen-pistil interactions.
• Fertilization - It occurs by double fertilization process to produce a diploid zygote and a triploid endosperm.
• Post-fertilization - It includes the development of embryo along with the endosperm to form the seed.

Important Points

• In an angiosperm, each pollen has two male gametes and are carried to the pistil by various pollinating agents.
• The pollen lands on the stigma and there is a chemical interaction between the pollen and the stigma to determine self-compatibility.
• When the compatible pollens are recognized, the pollen tube grows towards the ovule carrying the male gametes.
• Depending on the entry position of the pollen tube into the ovule, the fertilization process is divided into 3 types:
• Porogamy - The pollen tube enters the ovule and the embryo sac through the micropylar end and is the most common type. E.g.- Lily.
• Chalazogamy - The pollen tube enters the ovule from the chalazal end but enters the embryo sac from the micropylar end. E.g.- Casuarina.
• Mesogamy - The pollen tube enters through the funiculus or integuments but the entry into embryo sac is through micropylar end. E.g.- Cucurbita.

Key Points

• Cinchona is a flowering plant of the family Rubiaceae.
• It is native to the Andes of South America.
• The bark is used for medicinal purposes.
• The bark is dried to obtain alkaloids like quinine.
• The features of Cinchona bark are:
• Colour - Yellowish to brownish.
• Odour - Slight and distinctive.
• Taste - Highly bitter and astringent.
• Major Constituents - Quinine, Hydroquinine, Cinchonine.
• Pharmacological Properties - Antimalarial, antipyretic, stomachic, anti-inflammatory, anti-obesity, antioxidant, anti-cancer.
• Uses - Quinine is used in treatment of malaria, increasing appetite,  Quinidine is used to prevent arhythmias.

Additional Information

• Root of Rauvolfia - It contains 'reserpine' which is an anti-psychotic and anti-hypertensive drug used to treat hypertension.
• Seeds of Ricinus communis - These are castor beans that yield castor oil, but is also the source of a toxin called 'ricin' which is released when chewed and swallowed.
• Atropa belladona - It is a medicinal herb that yields 'atropine', which can be toxic as well as hallucinogenic.

Key Points

• The adult pancreas contains islets of Langerhans which act as endocrine tissue.
• The islets of Langerhans are named after the German physicist Paul Langerhans who first described them in 1869.
• They are clusters of endocrine cells that comprise less than 2% of the entire pancreas.
• The adult pancreas has about 1 million islets, with each islet having 3000 cells.

Important Points

• The islets are composed of 4 cell types:
• A (alpha) cells - are located on the periphery, constitute about 10% of islet cell mass and secrete glucagon.
• B (beta) cells - are located centrally within the islet, constitute about 70% of islet and secrete insulin.
• D cells - are evenly distributed within the islet, constitute about 5% of islet cell mass and secrete somatostatin.
• F cells - constitute about 15% of islet cell mass and secrete pancreatic polypeptide (PP).
• The D2 cells specifically secrete vasoactive intestinal peptide (VIP) which is also a polypeptide.
• Thus, the D2 and F cells together secrete the polypeptides.

Additional Information

• The functions of pancreatic hormones and polypeptides are as follows:
• Insulin - It regulates carbohydrates, fats and protein metabolism by promoting uptake of glucose and amino acids by cells and inhibiting breakdown of fats.
• Glucagon - It is antagonistic to insulin in function as it favours release of glucose from liver and fatty acids from fat tissues.
• Somatostatin - It inhibits gastrointestinal, endocrine and pancreatic secretions.
• PP - It inhibits exocrine secretion of pancreas.
• VIP - It induces smooth muscle relaxation in the digestive tract and controls secretion of water and salts during digestion.